Asunto(s)
Sistema Nervioso Autónomo/diagnóstico por imagen , Isquemia Encefálica/diagnóstico por imagen , Accidente Cerebrovascular Isquémico/diagnóstico por imagen , Cardiomiopatía de Takotsubo/diagnóstico por imagen , Tálamo/diagnóstico por imagen , Terapia Trombolítica/métodos , Anciano , Isquemia Encefálica/complicaciones , Isquemia Encefálica/terapia , Electrocardiografía/métodos , Humanos , Accidente Cerebrovascular Isquémico/complicaciones , Accidente Cerebrovascular Isquémico/terapia , Masculino , Cardiomiopatía de Takotsubo/etiología , Cardiomiopatía de Takotsubo/terapiaRESUMEN
Sixty New Zealand White weaned rabbits were divided into three groups and subjected to different dietary treatments: a standard diet for the control (C), a standard feed supplemented with 10% of plain olive leaves (OL) and a standard feed supplemented with 10% of selenium-fortified olive leaves (100â¯mg/L of foliar spray sodium selenate solution; SeOL). The productive performance was recorded at the time of slaughter (after 35â¯days); the carcass and meat traits were determined and estimated indexes of fatty acid metabolism were calculated. No significant differences were found on the rabbit productive performance and the physical-chemical characteristics of the meat. Both group of rabbits on the enriched diet showed leaner and thinner carcasses and a higher meat concentration of oleic acid. The estimated index of Δ5â¯+â¯Δ6-desaturase, starting from n-6 fatty acids, was lower in both groups supplemented with leaves. The use of selenium-fortified olive leaves, positively affected the lipid oxidative stability of rabbit meat.
Asunto(s)
Alimentación Animal , Ácidos Grasos Insaturados/metabolismo , Carne/análisis , Desarrollo de Músculos , Olea/química , Hojas de la Planta/química , Ácido Selénico/administración & dosificación , Aerosoles , Agricultura/economía , Algoritmos , Alimentación Animal/economía , Animales , Biofortificación , Femenino , Calidad de los Alimentos , Humanos , Residuos Industriales/análisis , Residuos Industriales/economía , Italia , Masculino , Carne/economía , Músculo Esquelético/crecimiento & desarrollo , Músculo Esquelético/metabolismo , Valor Nutritivo , Olea/crecimiento & desarrollo , Olea/metabolismo , Hojas de la Planta/crecimiento & desarrollo , Hojas de la Planta/metabolismo , Conejos , Ácido Selénico/metabolismo , Aumento de PesoRESUMEN
AIM: Bacteria levels of necrotic teeth are greatly reduced after endodontic treatment procedures but the presence of persisting microorganisms leads to continuous efforts to develop materials with antimicrobial properties. The purpose of the study was to determine the antimicrobial activity of polyethylenimine (PEI) against common bacteria and yeasts, regarding planktonic cells and biofilm, and to clarify its antimicrobial mechanism of action through flow cytometry. MATERIALS AND METHODS: The antibiofilm and antimicrobial effect of PEI was determined against Enterococcus faecalis, Staphylococcus aureus, Escherichia coli and Candida albicans strains using reference protocols. The effect of PEI was evaluated regarding adhesion, biofilm formation and biofilm disaggregation. In order to understand PEI cellular effects flow cytometric analysis was performed with different fluorescent markers. RESULTS: It was verified that minimal inhibitory concentrations (MIC) values and minimal lethal concentrations (MLC) obtained for PEI were similar and ranged between 50 and 400 mg/l, proving the microbicidal and fungicidal activity of this compound. Antibiofilm activity was also proved for all the microorganisms. Severe lesion of the membrane and cell depolarization was demonstrated. CONCLUSION: Polyethylenimine showed antimicrobial and antibiofilm activity against microorganisms often associated with apical periodontitis. CLINICAL SIGNIFICANCE: Theoretically, prolonging the antibacterial effects of materials used in endodontics may be interesting to help prevent reinfection and possibly to affect residual bacteria that survived the treatment procedures.
Asunto(s)
Antiinfecciosos/farmacología , Biopelículas/efectos de los fármacos , Polietileneimina/farmacología , Candida albicans/efectos de los fármacos , Endodoncia/métodos , Enterococcus faecalis/efectos de los fármacos , Escherichia coli/efectos de los fármacos , Humanos , Pruebas de Sensibilidad Microbiana , Staphylococcus aureus/efectos de los fármacosRESUMEN
PURPOSE: In this work, an alumina scaffold was produced through a new method to be used in a near future as a bone substitute. METHODS: In vitro and in vivo studies were performed in order to characterize the mechanical and biological properties of the scaffold. RESULTS: The results obtained showed that this scaffold has high mechanical resistance and a porous surface that allows human osteoblast cells to adhere and proliferate. The in vivo studies revealed no systemic reaction. CONCLUSIONS: The alumina scaffold produced herein has the mechanical and biological properties that are compatible with its application in bone therapy.